Laboratory / cell studycited by the Tayebat archiveabstract only checked A-WHT-17 standard review
How gluten protein binds gut cells
Gliadin induces an increase in intestinal permeability and zonulin release by binding to the chemokine receptor CXCR3.
Overview
Lammers KM et al.. Gastroenterology 2008 · PMID 18485912 · DOI 10.1053/j.gastro.2008.03.023
Gliadin binds a receptor and increases permeability in mouse tissue.
Does the study support the specific claim? Unclear · Methodological quality: moderate
Plain-language summary
Study explained simply
- What did the researchers want to find out?
Mechanism
- How was the study done?
Lab
- What was actually found?
CXCR3 binding
- What do the results mean in everyday life?
Lab mechanism
- What are the study's limitations?
No clinical data; COI
- What does it say about Elawady's specific claim?
Does not show harm in people
- What can explicitly not be concluded from this study?
Clinical harm
Scientific in-depth review
1. Full reference
- Authors
- Lammers KM, Lu R, Brownley J, Lu B, Gerard C, Thomas K, Rallabhandi P, Shea-Donohue T, Tamiz A, Alkan S, Netzel-Arnett S, Antalis T, Vogel SN, Fasano A
- Journal
- Gastroenterology
- Year
- 2008
- Identifier
- PMID 18485912 DOI 10.1053/j.gastro.2008.03.023 · PMC2653457
- Verification
- verified – PubMed E-utilities efetch, 2026-10-25
- Retracted
- no
- Corrections
- none
- Extent checked
- abstract only
- Conflicts of interest and funding
COI: two authors (S.N.V., A.F.) have a financial relationship with Alba Therapeutics; NIH grants.
2. Research question
Molecular basis of gliadin-mucosa interaction
3. Study design and population
- Study design
- Laboratory / cell study – In vitro, mouse ex vivo, human biopsies
- Population
Cell lines, mice, biopsies
- Country
USA
- Period
not stated
- Sample size
not stated
4. Methods and endpoints
- Exposure / intervention
Gliadin
- Comparator
CXCR3-/- mice
- Endpoints
Binding, zonulin, permeability
- Statistical methods
Lab
5. Actual results
- Results
Gliadin binds CXCR3; CXCR3 elevated in active coeliac disease and normalised on gluten-free diet; gliadin increased zonulin and permeability in wild-type but not CXCR3-/- mouse intestine.
- Absolute risks
not applicable
- Authors' conclusion
Gliadin binds CXCR3 and leads to MyD88-dependent zonulin release and permeability.
6. Strengths
- Mechanistic
7. Weaknesses
- Lab; COI
8. Risk of bias
- Orienting tool
- none applicable
- Type of assessment
- simplified assessment (abstract only)
- Full formal assessment
- no
- Overall risk of bias
- not assessable
- Rationale
COI noted.
9. Assessment of the statistical interpretation
Not applicable.
10. Relevance to the specific claim
Mechanistic for C-WHT-09.
11. Independent contrary findings
12. Transferability to Germany and Europe
13. Overall methodological judgement
Lab.
| Multidimensional assessment | |
|---|---|
| Authenticity of the source | verified |
| Methodological quality | moderate |
| Risk of bias | not assessable |
| Precision of results | not applicable |
| Directness | very indirect |
| Reproducibility | partly replicated |
| Consistency with the overall evidence | unknown |
| Regional transferability | unknown |
| Adequacy of the interpretation (by the archive) | not supported by the study |
14. Open questions
15. Linked fact checks
Use by the Tayebat archive
Note: according to the archive, the studies were compiled by the archive operators. Whether Dr. Elawady himself cited this study is not established.
- What the archive says about the study
Listed in bibliography only.
- D. What does the archive derive from it?
Implies gluten harm.
- E. Is this derivation scientifically justified?
Not justified for general population.
- Adequacy of the interpretation (by the archive)
- not supported by the study