Measurement / residue studyindependent cross-checkabstract checked I-FAT-106 standard review

Some Egyptian olive oil contained illegal dyes

Optimization and validation of a rapid LC-MS/MS method for the detection and quantification of illegal dyes in olive oil from Egyptian markets.

Overview

Ragab M et al.. Food Chem 2025 · PMID 40446662 · DOI 10.1016/j.foodchem.2025.144924

Sudan Yellow in 5% of 870 samples; other dyes rarer.

Does the study support the specific claim? Partly · Methodological quality: moderate

Original publication

Plain-language summary

Study explained simply
What did the researchers want to find out?

Detect banned dyes in olive oil.

How was the study done?

Lab survey.

How many people, animals or samples were studied?

870 samples.

What was actually found?

Dyes in a minority.

How large was the observed effect?

Up to ~6% positive.

What do the results mean in everyday life?

Buy olive oil from reliable sources.

What are the study's limitations?

Only dyes tested.

What does it say about Elawady's specific claim?

Partly supports adulteration concern.

Do the results apply to Germany and Europe?

Egypt.

What can explicitly not be concluded from this study?

Cannot show olive oil itself is unhealthy.

Scientific in-depth review

1. Full reference

Authors
Ragab M et al.
Journal
Food Chem
Year
2025
Identifier
PMID 40446662 DOI 10.1016/j.foodchem.2025.144924
Verification
verified – PubMed efetch 2026-10-26
Retracted
no
Corrections
none
Extent checked
abstract only
Conflicts of interest and funding

No competing interests declared.

2. Research question

Are banned dyes present in olive oil sold in Egypt?

3. Study design and population

Study design
Measurement / residue study – Analytical method validation plus market survey
Population

Olive oil samples from Egyptian local markets

Country

Egypt

Period

not stated in abstract

Sample size

870 samples

Inclusion and exclusion criteria

Market samples

4. Methods and endpoints

Exposure / intervention

Banned (Sudan) dyes

Comparator

Legal absence

Endpoints

Detection and concentration

Statistical methods

Method validation per CIR (EU) 2021/808

5. Actual results

Results

Detected: Sudan Yellow 5%, Sudan I 0.9%, Sudan III 0.2%, Sudan Red G 0.1%, Sudan II 0.1%, Sudan Red B 0.1% of samples. LOD 0.1-0.3 ug/kg.

Absolute risks

not applicable

Authors' conclusion

The method is applicable; banned dyes were found in Egyptian market olive oil.

6. Strengths

  • Large Egyptian sample
  • Validated method

7. Weaknesses

  • Only dyes tested (not oil substitution or other fraud)
  • Sampling frame not described in abstract

8. Risk of bias

Orienting tool
none applicable
Type of assessment
simplified assessment (abstract only)
Full formal assessment
no
Overall risk of bias
not assessable
Rationale

Analytical survey.

9. Assessment of the statistical interpretation

At most about 6.4% of samples contained any tested dye (sum of shares; stats/C-FAT-04_oils.py).

10. Relevance to the specific claim

Confirms that olive oil adulteration occurs on the Egyptian market, supporting a concern about quality, but most samples were free of the tested dyes, contradicting 'it is all fake'.

11. Independent contrary findings

Other adulteration types not tested.

12. Transferability to Germany and Europe

Egypt-specific; EU has its own fraud controls.

13. Overall methodological judgement

Partial support for the adulteration concern; not a reason to consider authentic olive oil harmful.

Multidimensional assessment
Multidimensional assessment
Authenticity of the sourceverified
Methodological qualitymoderate
Risk of biassome concerns
Precision of resultsmoderate
Directnessindirect
Reproducibilityunknown
Consistency with the overall evidenceunknown
Regional transferabilityhigh
Adequacy of the interpretation (by the archive)not applicable

14. Open questions

Extent of oil-substitution fraud in Egypt.

15. Linked fact checks