Cross-sectional studyindependent cross-checkabstract checked I-EGG-129 standard review

One farm's eggs often contained antibiotic residues

Risk factors associated with Salmonella prevalence, its antibiotic resistance, and egg antibiotic residues in the layer farming environment.

Overview

Sornplang P et al.. Vet World 2022 · PMID 35497971 · DOI 10.14202/vetworld.2022.543-550

On a single farm, streptomycin residues were found in 36% of egg yolks by screening test.

Does the study support the specific claim? Partly · Methodological quality: low

Original publication

Plain-language summary

Study explained simply
What did the researchers want to find out?

Study Salmonella and residues on a layer farm.

How was the study done?

Repeated sampling on one farm.

How many people, animals or samples were studied?

488 samples; 144 eggs.

What was actually found?

Frequent residues; Salmonella in the environment.

How large was the observed effect?

Streptomycin 36.1%.

What do the results mean in everyday life?

Bad farm practice can leave residues; it shows why controls are needed.

What are the study's limitations?

One farm; screening test.

What does it say about Elawady's specific claim?

Supports occurrence under poor practice.

Do the results apply to Germany and Europe?

Low.

What can explicitly not be concluded from this study?

It cannot show consumer harm or typical levels.

Scientific in-depth review

1. Full reference

Authors
Sornplang P et al.
Journal
Vet World
Year
2022
Identifier
PMID 35497971 DOI 10.14202/vetworld.2022.543-550
Verification
verified – PubMed efetch 2026-10-26; Crossref works API 2026-10-26
Retracted
no
Corrections
none
Extent checked
abstract only
Conflicts of interest and funding

not stated in abstract

2. Research question

What are Salmonella prevalence, resistance and egg antibiotic residues on a closed-housing layer farm?

3. Study design and population

Study design
Cross-sectional study – Repeated cross-sectional study on one battery-cage layer farm
Population

One laying farm with battery-cage closed housing (country not stated in abstract)

Country

Not stated in abstract

Period

not stated in abstract

Sample size

488 samples; 144 eggs for residue testing

Inclusion and exclusion criteria

not detailed in abstract

4. Methods and endpoints

Exposure / intervention

Farm practices

Comparator

Not applicable

Endpoints

Salmonella prevalence; residues by European Four Plate Test

Statistical methods

Logistic regression for risk factors

5. Actual results

Results

Residues in egg yolks: streptomycin 36.11%, enrofloxacin 11.81%, tetracycline 7.64%; Salmonella on pen floors 65.5%, egg sizing machine 52.5%, eggshells 15%.

Absolute risks

not reported

Authors' conclusion

Egg antibiotic residues may relate to treatment of resistant Salmonella and medicated feed with inappropriate withdrawal time.

6. Strengths

  • Repeated sampling

7. Weaknesses

  • Single farm
  • Screening test (Four Plate Test), no concentrations

8. Risk of bias

Orienting tool
none applicable
Type of assessment
simplified assessment (abstract only)
Full formal assessment
no
Overall risk of bias
high
Rationale

Single farm; not representative.

9. Assessment of the statistical interpretation

Descriptive; cannot be generalised.

10. Relevance to the specific claim

Shows that poor withdrawal practices can leave residues in many eggs on an individual farm.

11. Independent contrary findings

National data show low contamination (I-EGG-128, I-RES-07).

12. Transferability to Germany and Europe

Low.

13. Overall methodological judgement

Illustrates a mechanism; not representative.

Multidimensional assessment
Multidimensional assessment
Authenticity of the sourceverified
Methodological qualitylow
Risk of biashigh
Precision of resultslow
Directnessdirect
Reproducibilityunknown
Consistency with the overall evidenceunknown
Regional transferabilitylow
Adequacy of the interpretation (by the archive)not applicable

14. Open questions

Concentrations and health relevance.

15. Linked fact checks