Measurement / residue studyindependent cross-checkabstract only checked I-RES-09 standard review

Hormones in Egyptian meat (ELISA)

Hormonal residues in chicken and cattle meat: A risk threat the present and future consumer health.

Overview

Kamaly HF & Sharkawy AA. Food Chem Toxicol 2023 · PMID 37956705 · DOI 10.1016/j.fct.2023.114172

A small study reported high hormone levels, but its method cannot tell natural from added hormones.

Does the study support the specific claim? Unclear · Methodological quality: low

Original publication

Plain-language summary

Study explained simply
What did the researchers want to find out?

Measure hormones.

How was the study done?

ELISA.

How many people, animals or samples were studied?

148 samples.

What was actually found?

High estimated intake.

How large was the observed effect?

HI > 1.

What do the results mean in everyday life?

Data gap in Egypt.

What are the study's limitations?

Weak method.

What does it say about Elawady's specific claim?

Unclear.

Do the results apply to Germany and Europe?

Low.

What can explicitly not be concluded from this study?

Illegal use.

Scientific in-depth review

1. Full reference

Authors
Kamaly HF & Sharkawy AA
Journal
Food Chem Toxicol
Year
2023
Identifier
PMID 37956705 DOI 10.1016/j.fct.2023.114172
Verification
verified – PubMed efetch 2026-10-25
Retracted
no
Corrections
none
Extent checked
abstract only
Conflicts of interest and funding

Declaration of competing interest The authors declare that there is no conflict of interest.

2. Research question

Hormone residues in Egyptian chicken and cattle meat

3. Study design and population

Study design
Measurement / residue study – ELISA measurement of estradiol, progesterone, testosterone in retail chicken and beef with risk assessment
Population

92 chicken, 56 beef samples

Country

Egypt (Assiut)

Period

2022

Sample size

148

4. Methods and endpoints

Comparator

ADI

Endpoints

Hormone levels; EDI; HI

Statistical methods

ELISA

5. Actual results

Results

EDI of estradiol exceeded ADI in almost all samples; HI of estradiol and testosterone > 1 in all samples; no Egyptian limits set.

Absolute risks

not reported

Authors' conclusion

Monitoring urgently needed.

6. Strengths

  • Addresses data gap

7. Weaknesses

  • ELISA cannot distinguish endogenous from administered hormones
  • Small
  • Unusual risk framework

8. Risk of bias

Orienting tool
none applicable
Type of assessment
simplified assessment (abstract only)
Full formal assessment
no
Overall risk of bias
high
Rationale

Method limits.

9. Assessment of the statistical interpretation

10. Relevance to the specific claim

C-RES-04.

11. Independent contrary findings

12. Transferability to Germany and Europe

Low.

13. Overall methodological judgement

Weak evidence; flags a data gap.

Multidimensional assessment
Multidimensional assessment
Authenticity of the sourceverified
Methodological qualitylow
Risk of biashigh
Precision of resultslow
Directnessdirect
Reproducibilitynot replicated
Consistency with the overall evidenceunknown
Regional transferabilitylow
Adequacy of the interpretation (by the archive)not applicable

14. Open questions

Confirmatory LC-MS/MS data.

15. Linked fact checks